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AG, AGS, and EZ Series Universal Testers INDUSTRIESCeramics, Metals, Mining Clinical research, Forensics Electronics, Electronic Environment hplc chromatography Food, Beverages Machinery, Automotive New Energy Petrochemical, Chemical Pharmaceutical, Life Science LITERATURESUMMARY Sitemap placeholder SUPPORTSitemap placeholder NEWS/EVENTSNEWS PRODUCT INFORMATION NEW APPLICATIONS You are hereHOME > SERVICES & http://www.interchromforum.com/html/ql_err_hplc.html SUPPORT > HPLC:Tips for Daily Analysis > Quantitation Error Factors in Sample Preparation Tweet Quantitation Error Factors in Sample Preparation HPLC: Tips for Daily Analysis The processes involved in analysis by HPLC can be categorized as sample preparation, injection, separation, detection, and data processing. Factors that can affect quantitation error are present in http://www.shimadzu.com/an/hplc/support/lib/lctalk/26/26lab.html all of these stages, but here we focus on factors in the sample preparation stage. Preparing Standard SolutionsThe tendency is to think of things too simplistically, but there are many factors to consider, such as those in Table 1, for example. Of these, one of the most important factors is (K) Adsorption of Target Components to the Container. In the example shown in Table 2, in some cases adsorption can be suppressed by selecting a different solvent or changing the container material. Adsorption is also discovered by not being able to achieve calibration curve linearity (or the line does not pass through the origin) when the solution is diluted. Another important factor is (N) Oxidation or Decomposition of Target Components. For example, since ascorbic acid is easily oxidized by dissolved oxygen or iron(III) ions in an aqueous solution, its concentration decreases over time. If this occurs, we recommend reducing the pH level or masking the iron ions with EDT

ChapterPractice of High Performance Liquid Chromatography Part of the series Chemical Laboratory Practice pp http://link.springer.com/content/pdf/10.1007/978-3-642-69225-3_2.pdf 65-108Quantitative Analysis in HPLCJürgen AsshauerAffiliated withHoechst AG, Werk Knapsack, Helmut UllnerAffiliated withHoechst http://www.ncbi.nlm.nih.gov/pubmed/3584387 AG Buy this eBook * Final gross prices may vary according to local VAT. Get Access Abstract The general object of analytics is to furnish information about the qualitative and quantitative composition of mixtures of substances. A quantitative statement on a lot liquid chromatography of material (product) should describe the true value of a property of this material as well as possible. Both the statement and the product may be traded and transmitted to a customer. In most cases this involves a flow of countervalues in the form of goods or currency or information. Page %P Close sources of error Plain text Look Inside Chapter Metrics Provided by Bookmetrix Reference tools Export citation EndNote (.ENW) JabRef (.BIB) Mendeley (.BIB) Papers (.RIS) Zotero (.RIS) BibTeX (.BIB) Add to Papers Other actions About this Book Reprints and Permissions Share Share this content on Facebook Share this content on Twitter Share this content on LinkedIn Supplementary Material (0) References (21) References1.Currie, L.A.: “Scientific Uncertainty And Societal Decisions: The Challenge To The Analytical Chemist”. Analytical Letters, 13(Al), 1–31 (1980)CrossRef2.Kratochvil, B., Wallace, D., Taylor, J.K.: “Sampling for Chemical Analysis.” Anal. Chem. 56, 113R–129R (1984)CrossRef3.“General Guideline for the Establishment, Maintenance, and Distribution of Chemical Reference Substances.” WHO, Technical Report Series Nr. 681 (1982)4.Wiese, Martin, Hermansson: Chromatographia 15, 737–742 (1982)CrossRef5.Glajch, J.L., Kirkland, J.J.: Anal. Chem. Vol. 55, 319A–326A (1983)CrossRef6.McDowell, L.M., Barber, W.E., Carr, P.W.: Anal. Chem. 53, 1373–1376 (1981)CrossRef7.Campbell, J.E., Hewins, M., Lynch, R.J., Shrewsbury D.D.: Chromatographia 16, 162–165 (1982)CrossRef8.Eichelberger, W., Günzler, H.: „Analytiker Taschenbuch Bd 1“, Ed. H. Kienitz et al. Springer Verlag,

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